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Aligned Solexa sequencing was merged to confirm assembly and also confirm sequence in 454 only regions.
In principle, the 'best hit' was considered the ortholog but, additionally, the difference between the best hit and the second-best hit within a species and the proportion of positive amino acid (aa) matches were taken into account to confirm sequence identity.
All reactions were performed in duplicate to confirm sequence differences.
The mutagenesis positive colonies will be sequenced directly to confirm sequence alternations.
If amplified by PCR, be sure to confirm sequence to exclude Taq error.
The protein size limit is required to provide sufficient phylogenetic information for meaningful control trees, which are needed to confirm sequence orthology.
Similar(42)
Two researchers confirmed sequence and genotyping analyses.
Confirmed sequences are available online [GenBank: EU876805 - EU876827].
Confirmed sequences were aligned using MEGA 4 software [ 26].
PCR products were sequenced to confirm the sequence.
Sequences in GenBank confirmed our sequence.
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