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The experiments reported here were designed to confirm rigorously or not that this irregular tryptic cleavage occurs in polyubiquitins and, by implication, in proteins conjugated with ubiquitins.
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Thus, in individualized medicine candidate associations limited to certain patient subgroup/marker/outcome combinations not only benefit fewer patients, but should also be confirmed more rigorously in independent experiments and populations.
If a second instance of a similar unmapped sequenced gene occurs, the best reciprocal match approach should be applied to rigorously confirm whether it is, in fact, the same as the gene previously identified.
Further studies, with larger and best characterized squamous cervical cancer series, are required to rigorously confirm these results, as well as evaluate possible associations with survival.
Therefore, to rigorously confirm that the Y21 INV construct had been modified with a GPI anchor rather than secreted into the periplasm, the membrane and cell wall fractions of the Y21-encoding strain were separated by detergent solubilization.
These less favourable estimates confirm the discrepancy between rigorously planned protocol trials and real-life clinical practice.
The validity and reliability of the BARSTL survey has not been rigorously confirmed outside of the initial design and validation study by Sampson and Grooms (2013).
This relationship, which I have suspected for many years based on casual observation, was recently confirmed by a rigorously researched study that appeared in the Journal of Finance.
Twin status was rigorously confirmed.
In another study that rigorously confirmed the bipolar diagnosis, 25.6% of psychiatric outpatients with bipolar I and 50.5% with bipolar II disorder were not diagnosed [ 8].
We rigorously confirmed orthologies of the snake sequences to their homologs of other vertebrates by constructing molecular phylogenetic trees with the neighbor-joining method [ 44] using XCED in which the alignment algorithm MAFFT is implemented [ 45] and with the maximum-likelihood method using PhyML [ 46].
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