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Our data additionally confirm responsiveness of cells with FOP ACVR1 mutations to both BMP and Activin A ligands.
For each round of infection, control wells on separate plates were studied in medium alone to confirm low basal fluorescence and after exposure to 2.5 ng/mL IFNγ to confirm responsiveness to a stimulus.
It is highly recommended to use multiple independent anchors and to examine and confirm responsiveness across multiple samples.
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Thus, the elasticity at each age group is calculated to confirm the responsiveness of dependent variables to age.
To further confirm the responsiveness of Arabidopsis HSFs to AZC and HS under our test conditions, we analyzed transcript levels of Arabidopsis HSFs under AZC and heat treatments (data not shown).
To confirm cell responsiveness, 0.2 µg of a monoclonal anti-IgE antibody (clone BE5; EurobioSciences, Friesoythe, Germany) served as CCD-independent positive control.
Our observations confirm the responsiveness of this infection to antiviral therapy.
Further investigation is required to confirm the responsiveness of the Emotional score.
To further confirm androgen responsiveness of SOCS2, we expected that androgen withdrawal would result in a diminished SOCS2 expression.
However, an interventional study to define a responder threshold and confirm the responsiveness of the SOBDA was conducted and is reported separately [ 26].
To confirm p53 responsiveness of the identified p53 REs we first applied a well-established quantitative reporter assay in the budding yeast Saccharomyces cerevisiae[ 37, 43].
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