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Gene Ontology analyses of miR-145 responsive genes confirm regulation of genes involved in cell death, cell cycle regulation and cancer.
To confirm regulation of four selected genes, the abundances of the mRNAs were analysed with qPCR.
In comparison, mmu-miR-503* could be detected, however, we could not confirm regulation.
To confirm regulation of PI3 kinase signaling in HFD-fed mice, we measured PI3K and Akt activities in the liver.
Because expression levels of BTC on the human array were relatively low, qRT-PCR was used to confirm regulation in the human.
Depicted in Figure 3 are immunohistochemistry stainings performed with EGF transgenic livers to confirm regulation and predominant cytoplasmic expression of arginase II.
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The paper also confirms regulation overlaps and the need for the systematic approaches proposed by [45, 47, 55].
We additionally confirmed regulation of some genes by immunohistochemistry and therefore demonstrate good concordance between gene regulation and coded protein.
However, mRNA expression of the splice variant Zfhep2 was higher in DA.PVG-Eae23 (p<0.018) compared to DA (Figure 8D), confirming regulation by the Eae23 locus.
Luciferase assays confirmed regulation of Notch signalling by Robo.
In each instance, the qRT-PCR confirmed regulation trends observed in the microarray analysis.
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