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The experimental results show the DSCM can confirm orientation function of the optical mouse, and have a good agreement with the simulation results, and the resolution we obtained is higher than the resolution of mouse on the market.
The construct was sequenced to confirm orientation and accuracy.
The insert was transferred into pHELLSGATE12 [ 41] through LR reaction and the construct was sequenced to confirm orientation of the inserts.
Semithin sections were stained with either toluidine blue, to confirm orientation, or para-phenylenediamine (PPD; MP Biomedicals Inc, Solon, OH) for quantification of myelinated axons (as described later).
The resulting plasmids pBOVP2, pWUVP1, and pKIVP1 were extracted from overnight culture with the QIAprepSpin Miniprep Kit (Qiagen, Hilden, Germany) and sequenced by standard techniques to confirm orientation and sequence identity of the plasmid insert.
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Firstly, we cloned the full-length human PEA3-encoding cDNA sequence into a mammalian expression vector, pcDNA4/TO, and confirmed orientation and fidelity by sequencing the whole insert (data not shown).
Good experiences with graphically oriented toolbox environment usage confirm correct orientation of the discussed software.
Besides, crystalline morphology and orientation were studied by wide and small angle X-ray scattering to confirm the orientation effect of the shear in the crystalline part of the material.
Several studies have been performed to confirm the orientation dependent adsorption of surfactant molecules [18, 19, 22].
The nanocrystal arrays were characterized by X-ray diffraction (XRD) to confirm the orientation of crystalline platinum islands with respect to the substrate.
To confirm the orientation of each transmembrane domain of FIT2 and FIT1 by an independent method, we used selective permeabilization of the plasma membrane in conjunction with indirect immunofluorescence microscopy to the FLAG epitope in each FNF construct.
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