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Transformants were also streaked on QDO medium supplemented with 40 µg/ml 5-bromo-4-chloro-3-indoxyl-α-d-galactopyranoside (X-α-Gal) to further confirm interaction of different co-transformants.
In contrast to the interactions between Rio2 and Nob1, and Rio2 and Dim1, reverse pulldowns do not confirm interaction of Rio2 with Dim2/Pno1 or Tsr1 (Table S1).
In order to further confirm interaction between δ-toxin and the host AMPs, tryptophan spectroscopy was performed on δ-toxin in the presence and absence of host AMPs.
In order to confirm interaction pairs (PPIs and IRs), pull-down experiments were performed as described in previous studies [19], [20], and the precipitates were analyzed by 10-17.5 10-17.5GE.
The data confirm interaction of those compounds with the rhodamine B efflux mechanism in zebrafish embryos that, as we show here, is Abcb4.
To confirm interaction of PEA3 with the Fgf-10 promotor region, we performed ChIP experiments using immortalised mouse lung endothelial cells.
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Plasmids were recovered from positive yeast clones and used subsequently to confirm interactions by independent cotransformations.
Next, a solid-phase binding assay was carried out to further confirm interactions between FtsZ and ClpX.
The system might be meaningful to confirm interactions (59).
If aiming for the identification of interactions of potential biological relevance, it is crucial to confirm interactions in the context of the full-length proteins.
β-Gal filter-assays also confirmed interaction in each case (Fig. 2, representative example).
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