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Therefore, these results altogether confirm functionally that p110α/p85β and p110α/p55γ are the largely predominant PI3-K isoform complexes in HIEC cells, in addition to indicating that both complexes contribute in the activation of Akt-1 and a consequent promotion/maintenance of survival in these cells.
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Infusion placement was confirmed functionally in later tests by reversible inactivation of the MD via intra-MD infusion of tetrodotoxin (TTX; 10 ng/side), and subsequently by direct histological examination.
The homology of the bacterial protein was confirmed functionally by DNA-binding assays.
Two putative caspase recognition and cleavage sites, each containing the ExD sequence [13], [29] [31], were identified in silico and then confirmed functionally in human BAG3.
All mutants were confirmed functionally active in a liposome-based F− efflux assay.
Each of these mutations are novel missense mutations, which are highly predicted to be deleterious, although this has not been confirmed functionally.
While genome-wide association studies in human and quantitative trait loci mapping in mice have provided evidence for multiple susceptibility genes, few of these have been confirmed functionally.
Several observations, based on the predictions in Table 2 and summarized in Figure 10, confirmed functionally separable roles for the CO/DA+ and FP clusters and mapped those roles on the 2-mechanism model.
The RT-PCR analysis confirmed functionally active endothelial cells in the cell sheet as evidenced by the positive expression of aquaporin 1, collagen IV, Na+-K+ ATPase, and FLK-1.
The notion that subgroups of ependymoma arise from regionally and developmentally distinct RGCs that have undergone transformation by subgroup-specific genetic mutations was further confirmed functionally in the case of supratentorial ependymomas.
Hinney, A. et al. Melanocortin-4 receptor gene: case-control study and transmission disequilibrium test confirm that functionally relevant mutations are compatible with a major gene effect for extreme obesity.
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