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In Figure 1 figure supplement 2B, we have further utilized candidate kinase activity profiles in a case study to confirm fidelity of our interpretation.
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Based on the model, a framework of Trusted Wireless Sensor node is presented consisting of two major sections which are platform security enhancement and Trusted Authentication protocol to enhance sensor nodes security features and confirm the fidelity of node joining the network respectively.
All genes were sequenced to confirm the fidelity.
Considering the specific environment in the ACA, caution is still needed for detailed clarification of the BGE in the BYH section and further attempts are necessary to confirm the fidelity of the BGE in this area.
The presence of the insert was confirmed by PCR and, finally, to confirm the fidelity of the sequence, DNA sequencing was performed.
To confirm the fidelity of the microarray data a subset of 15 genes was selected for validation using quantitative RT-PCR (Table 2).
Restriction mapping and DNA sequencing were used to confirm the fidelity of the PCR and the correct construction of the plasmid.
Sequences were verified to confirm construct fidelity and pBMN-HA-TGF-β1(a -IRES-eGFP wa -IRES-eGFPsfected into Phoenix a -IRES-eGFP a Calcium Phosphate Transfection kit (Invitrogen) for retroviral engineering of the NHPrE1.2 human prostate epithelial [24] and LNCaP cell lines.
Both constructs were fully sequenced to confirm plasmid fidelity.
Scanning electron microscopy (SEM, FEI Sirion) was used to confirm nanotopographical fidelity.
All connexin clones were sequenced to confirm the fidelity of the nucleotide sequence.
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