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To confirm efficient transfection, RT-PCR and western blotting were performed.
Blots were stained with methylene blue staining to confirm efficient transfer and equal loading.
To confirm efficient transfer the blot was also incubated sequentially with an antibody recognizing α-actin.
Northern blot analysis was carried out to confirm efficient transcription of all tested genes.
To confirm efficient Hsp90 inhibition, Hsp70 induction, a consequence of Hsp90 inhibition (15, 29), was also monitored (Fig. 4A and C).
CDK6 mRNA and protein levels were assessed after 24h, 48h and 72h to confirm efficient down-regulation (Additional file 3: Figure S3C,D).
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Wave attenuation mechanisms are also studied and transmission analysis confirms efficient wave filtering performance.
After confirming efficient local antigen expression that induced high and stable Interferon gamma (IFN-γ) production in intramuscular (i.m).
Moreover, we confirmed efficient DOX delivery into cancer cells and induction of the apoptotic capability in vitro.
Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7+ endosomes.
Gel electrophoresis and confocal microscopy confirmed efficient siRNA release from the (siRNA/PAMAM -PK NPsiRNA/PAMAM -PKrespoNPsve hydrolysis of the PK shell.
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