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Overall, our data confirm directional RNA deep sequencing as a more thorough method for analyzing transcriptional regulation in cyanobacteria and indicate that further studies using different environmental conditions and mutant strains will yield novel information about Anabaena gene regulation.
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Although this study did not include a measure of the efficiency of feeding (nectar consumed per unit time), it did confirm that directional selection on tongue length could potentially occur through an energetic benefit.
Finally, we implemented computer simulations to confirm that directional spatial genetic structure patterns can be produced by range expansions.
Other studies confirm this directional uncertainty, particularly for sleep and gastrointestinal disorders [ 4, 5, 74].
Subsequent computer simulations confirmed that directional patterns can be produced by expansion events.
The results of the present study show a somewhat stronger paradigm effect, with prospective estimates being on average 23% higher than retrospective ones, thus confirming the directional effect of the time estimation paradigm on the time ratio.
Investigation and additional, targeted intra- and interlab retesting confirmed these directional (higher) differences.
Small scale sequencing of 384 random clones confirmed the directional aspect of the inserts, the plant nature of the source cDNAs, and the frequency of positive clones.
It has been also experimentally confirmed that directional selection can perform differentially on size and metabolic rate between sexes in mammals [ 1, 2].
These results confirm that the directional assembly of BTAs is maintained upon incorporation of BTA-DNA monomers, both in the absence and presence of recruited proteins.
Simulation results confirm also that Directional BSS is very robust against localization errors.
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