Sentence examples for confirm alteration from inspiring English sources
Proteasome inhibition assays were conducted on cultured peripheral blood mononuclear cells (PBMCs) from ALS patients to confirm alteration of the Ubiquitin/Proteasome System (UPS).
Real-time PCR was also performed on the 12 prefrontal cortex samples to confirm alteration of the miR-132/-212 cluster, validating the downregulation of the four miRNAs (padj = 0.0005, ANOVA corrected by the BH procedure) (Fig 4D).
To confirm alterations in sulfation more directly, we performed immunostaining for C4S and C0S.
This methodological problem is underlined by the fact that Tanaka et al. (2006) could not confirm alterations of G-CSF levels by this approach.
In order to confirm alterations in protein expression, as identified by label-free mass spectrometry, immunoblot analysis using a panel of antibodies against select proteins was employed.
We were able to confirm alterations in 87% of the genes tested in MECP2-Tg mice and in 68% of the genes tested in Mecp2-null mice (P < 0.05) (Table 1).
Our data confirm alterations of the relationship between cardiac sympatho-vagal activity and delta power in patients suffering from severe SAHS, even in the absence of cardiovascular events or significant changes in HRV components.
We confirm alterations in six genes previously associated with prostate cancer (MAP3K7, MELK, RCBTB2, ELAC2, TPD52, ZBTB4), and also identify 94 genes not previously linked to prostate cancer progression that would not have been detected using either transcript or copy number data alone.
Semi-quantitative multiplex PCR (SI7) confirmed alteration of mRNA levels.
For confirmed alterations in tumor samples, PCR and sequencing were performed in matched normal samples to determine whether an alteration was somatic or germline.
Western blotting experiments confirmed alterations in glycolytic and oxidative metabolism, as we observed decreased expression of ENO3, GPD1, NDUFS2 and UQCRFS1, in total extracts of old muscle compared to adult muscle.
