Previous data have shown that MT1 and MT2 are ubiquitously expressed in both species whereas MT3 and MT4 present a confined expression in human and mouse brain [43] and in mouse epithelial tissue [17] respectively, although data regarding expression of MT4 in human tissues is still missing in the literature.
The antigenic epitope within the non-collagenous-1 (α3 NCI) domain of type IV collagen in the GBM is well defined, and the confined expression of this collagen to glomerular and alveolar basement membrane leads to organ specificity of the disease.
Moreover, we validated reduced and confined expression for some of their secondary response factors during the subsequent pathogen challenge.
We test whether negative feedback from Ime2 to Ime1 is responsible for confining expression of early meiotic proteins to a narrow window.
Unravelling the developmental profile of the EBJC requires a high anatomical resolution that matches the spatially confined expression of this junctional complex.
We then confined expression of these RNAi transgenes against Met, gce, or their target Kr-h1 to adult intestinal progenitors using esgReDDM.
At first glance, this might seem surprising since genes with the most confined expression levels could be thought of as being most sensitive to perturbations affecting gene expression, for example, changes in gene dose.
Moreover, by identifying genes or transcriptional programs displaying a confined expression only to the leukemic cells and with absent expression in normal hematopoietic cells, new candidates for targeted treatment may be identified.
This result is in agreement with earlier studies [ 5, 6], and demonstrates that the temporarily confined expression patterns of meiotically regulated genes enables association of peak expression or repression with a specific developmental stage in our experimental setup.
Alternative approaches include confining expression of proteins to selected cell compartments [ 18- 20], targeting transgene expression to tissues with low metabolic turnover [ 21, 22], co-expression of a specific recombinant protease inhibitor [ 15, 23], or by fusion to stabilising protein domains [ 24].
