The problem is further compounded by the very high excess of L-aa-tRNA over D-aa-tRNA in the cellular milieu and warrants a stringent D-configuration specificity to avoid depletion of L-aa-tRNA pool.
For each model and cross validation fold configuration, the sensitivity and specificity were recorded at the threshold at which both values are simultaneously maximized.
We predicted that a G107S substitution in TRBV13-2 TCR, would stabilize the CDR3β loop in configurations that retain antigen specificity, and could thereby increase TCR affinity for cognate ligand.
(1) 15(S -HETE waS -HETEd in lowas abundance and metabolized to 15-oxo-ETE, as expected formedts 15(S)-confinuration and the substrate specificity of 15-PGDH.
The molecular design of the diode was optimized to excite a fluorophore-conjugated antibody and tested on a protein microarray configuration with good sensitivity and specificity.
This probe configuration should give a higher specificity of probes for the identification of transcript variants and alternative splicing isoforms.
Independent estimates of the sensitivity and specificity for this configuration are presented in Supplementary Material, Methods S10, which compare well to consistency rates determined for PennCNV in a comparative study of CNV detection methods (46).
In particular, C domains succeeding an E domain are expected to show specificity towards the configuration (L or D) of the C-terminal residue that is bound at the donor site because the preceding E domain does not specifically catalyze the epimerization from L to D but provides a mixture of configurations.
Running Blastz with a contiguous seed of length 8 bases, see BZc1 (Table 1), had a lower sensitivity than PatternHunter, with a very similar percentage of the BACs in the total dataset positioned in the tail-to-tail configuration and therefore an increased specificity, i.e. fewer BACs with positions and a higher probability that the BESs position using Blastz are correctly positioned.
Consistency of the two test configurations is discussed and the specificity of the off-axis case is analyzed.
The specificity of the SN configuration was confirmed by Southern blot analysis and automated sequencing of the PCR product.
