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We have retained the label "CC", however, since we are confident of the assignment.
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Due to the inherent noisy nature of the transcriptome, the random errors of the experiments due to bias in library construction, amplification and sequencing efficiency (30 33), and the fact that it is not straightforward to discriminate between primary from processed transcripts, high reproducibility needs to be fulfilled in order to be confident of the TSSs assignment.
In the majority of cases, the recapitulation of the species phylogeny in these genes means that we can be highly confident of these assignments.
Moreover, one may be more confident in the assignment of SSRs where they amplify single polymorphic bands with sizes similar to the expected fragments from DNA derived from amphidiploid plants.
Thus we can be reasonably confident that the assignment is valid because the sum of the 10-year relative survival estimates for the two re-assigned groups is close to that for the original group with unknown stage (85.5%).
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Hence, we feel confident of the validity of our instrument.
In the absence of confident similarity scores, assignment of common function to different sequence families requires additional evolutionary and structural justifications.
We were confident in our assignment of the break-point between the 5' and 3' sections because CR1 and CR2 sequences were either identical or differed by a maximum of two mutations downstream of the breakpoint.
This target enrichment results in an increased read-depth of the targeted loci, a critical consideration for validating the existence of bona fide SNPs, for confident detection of appropriate assignment of alleles for heterozygous loci, and for diagnosis of the genomic origin of homeologous genes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com