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Exact(33)
Despite the importance of the approach to experimental biology, the confident assignment of reaction efficiency to the early cycles of real-time PCR reactions remains problematic.
Returning to the question that conservation officers might have about identifying the geographic origin of a specific animal as an example, this application requires a significant number of DNA markers and samples from different localities within the database to ensure a confident assignment of an unknown sample to a particular geography.
In subsequent years, other finds permitted a more confident assignment to Multituberculata.
Confident assignment of an individual to a cluster was determined to have failed if the probability associated with movement of a genotype from its optimum cluster to any other was greater than a Bonferroni-corrected critical alpha level.
Certain fossil forms would then be expected to show a mosaic of primitive and derived characters that indicates affinity to the greater clade Caprini+Hippotragini+Alcelaphini, but makes confident assignment to any one of these tribes difficult.
It measures the strength of support ("confidence") for the species branch: If there is no clear evidence for this, a confident assignment may not be possible by any barcoding assignment method, unless, perhaps, one is certain that all relevant haplotypes have been sampled (although see [42]).
Similar(27)
Class I sites were considered as confident assignments and used for all analyses in the study.
Instead, the PrP fragments with confident assignments are the same in TgPrP S3.F88W) mice and Tga20 mice expressing WT PrPC (G. Schmitt-Ulms, personal communication).
Remarkably, the gene content of B. aphidicola is a subset of the E. coli K-12 genome [ 3, 5], allowing nearly all Buchnera gene products to be assigned confident functional assignments.
Matching accurate mass and retention time of each ligand with the reference data are required for confident structural assignment.
Several methods rated the immunoglobulin-type fold most highly but with scores not high enough for confident fold assignment.
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