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"This game is all about confidence," Fox said, "and you need to have some success to be confident.
High confidence fox SNPs were selected using parameters described in the Methods section.
In total, 29,834 of these high confidence fox SNPs were localized in the dog genome and the inferred location of 29,368 of these SNPs on fox chromosomes was assigned using the comparative fox/dog map (Additional file 4, Figure S3).
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Mapping fox reads against the fox transcriptome assembly and the dog genome identified over 30,000 high confidence fox-specific SNPs.
After applying filtering criteria (see Methods section) 17,328 high-confidence fox SNPs were identified.
100 randomly selected high-confidence fox SNPs were mapped onto the dog genome sequence assembly (CanFam2) to identify their distribution among different types of genome sequence.
To establish parameters for filtering high-confidence fox SNPs (see Methods section) we selected a subset of SNPs for validation by Sanger sequencing.
Mapping fox reads against both the canine genome and the assembled fox transcriptome sequence identified 30,491 high-confidence fox-specific SNPs.
Overall, transcriptome sequencing from the prefrontal cortex of two fox individuals identified over 30,000 high-confidence fox-specific SNPs, fox orthologs of over 14,000 dog genes, and yielded new insights into potentially important differences in expression of genes in the pre-frontal cortex between tame and aggressive foxes.
"The pressure is now on management to sell investors on the 'plan' that gave the company so much confidence to ignore Fox's interest in acquiring them," said Michael Nathanson, media analyst with MoffettNathanson Research.
These studies with different designs verify that it is possible to improve confidence of blue foxes towards humans using selection.
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