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The quantification method [ 24] was conducted with modification.
The BA quantification method [ 23] was conducted with modification.
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CPP was conducted with modifications of our recent protocol [52].
Mitotic chromosome preparations and in situ hybridization were conducted with modifications to Kato et al. (2011) as described in Chester et al. (2012).
DNA isolation from male middle tibia, female abdomens, and whole pupae was conducted with slight modification to the protocol of Holehouse et al. [ 53].
A human sensory test was conducted with slight modifications [17].
The isolation and characterization of the microsatellite loci were conducted with slight modifications for enriched genomic libraries, described in Zane et al. [27].
Immunocytochemical analysis of GnIH was conducted with slight modifications of our previous method [19] on coronal sections at 40 µm thickness of five fixed human hypothalami.
Caspase inhibition assays were conducted, with minor modifications, as described by Margolin and colleagues [ 34].
The α-syn aggregation assay was conducted with minor modifications as published previously [ 38].
The tau aggregation assay was conducted with minor modifications as published previously for an α-synuclein aggregation assay [ 47].
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