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We have successfully conducted this procedure on >150 independent isofemale lines (data not shown).
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To prevent population collapse due to nutrient depletion, we replaced 10 ml of microcosm contents with sterile medium in each undisturbed community after 3 weeks [43]; we did not conduct this procedure in disturbed patches because nutrient medium was already being replaced through the disturbance process.
When conducting this procedure, we closely monitored birds for signs of distress (none of which were shown).
Cardiorespiratory fitness will be estimated using the PACER test which consists of an endurance-type shuttle run which will be conducted by program staff trained to conduct this procedure following standardized procedures [ 66].
"He's got quite significant rectal inflammation which is a result of colitis, so that immediately, this is a red flag to me about the way I'm going to conduct this procedure because of the potential risk of inflammation throughout the rest of the colon.
When conducting this procedure, the quadratic interaction term between genotype and time (i.e., (GT) ti) was removed prior to the linear interaction term between genotype and time (i.e., (GT) ti), which was removed prior to the main effect of genotype (i.e., G t).
In the Australian cohort, indications to conduct this procedure were primarily to exclude aneuploidy based on high risk findings at either first trimester screening (ultrasound measurement of fetal nuchal translucency and maternal biochemistry) or second trimester maternal serum screening (maternal biochemistry) with no apparent fetal structural anomalies.
An angiography conducted after this procedure, showed a diameter equal to the nearby normal segment.
On the sixth day, a transfer test (or probe test), which is a trial without the platform, was conducted; in this procedure, we counted the number of times that the mouse crossed the position where the platform had been.
Conduct this procedure for five minutes before retiring and for five minutes immediately after you wake.
During the conduct of this procedure, the tape-recorded versions and the Oriya and English transcripts were consulted repeatedly during the coding procedure to understand the full meaning of the text.
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