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Three different types of assays were conducted: primer extensions, single nucleotide incorporations, and primer extensions followed by LC-ESI-MS-MS sequencing.
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β-lactamase PCR and sequencing were conducted (primers listed in Table 1).
Triplex PCR (three pairs of primers with different dyes) was performed when conducting primer screening.
In the subsequent step, the insertion pol is replaced by another TLS pol to conduct primer extension past the lesion site.
The amplified fragments were mixed and then a second PCR was conducted using primer 1 of set 1 and primer 2 of set 2. The resultant PCR products were inserted into the SfiI site of the pBE-CD vector.
The amplified fragments were mixed and then a second PCR was conducted using primer 1 of set 1 and primer 2 of set 2. The resultant fragments were digested using the BamHI restriction enzyme.
Michel Willemin, the chief executive of Asulab, a research and development laboratory owned by Omega's parent company, the Swatch Group, conducted a primer on magnetism that led with a slide presentation: "Permanent Magnets & You".
PCR was conducted employing primer pair.
The study was conducted using primer hpt (forward) 5'-GATGCCTCCGCTCGAAGTAGCG-3', (reverse) 5'-GCATCTCCCGCCGTGCAC-3', gus-A (forward) 5' GCCATTTGAAGCCGATGTCACGCC-3' GCCATTTGAAGCCGATGTCACGCC-3TGandGTCGCAGAAC-3'.
Quantitative PCR was conducted with primer pairs centered 500 to 700bp downstream of each gene.
All prevalence modeling analyses were based on zero-adjusted Bray-Curtis similarity matrices [47] and conducted in PRIMER v6 [48] and PERMANOVA+ [45].
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