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JJ designed the study and conducted mutation validations.
Among the 27 tumors, we conducted mutation validation for 26 tumors using the combination of Lung/OncoCarta panels (http://www.sequenom.com/).sequenom.com/
We conducted mutation analyses of critical and apparent amino acid residues of influenza A(H7N9) virus isolates.
In order to identify disease-causing mutations in the OPTN locus, we conducted mutation screening of the coding exons of OPTN in 200 PDB patients, but results showed no mutations in the protein-coding region (data not shown).
Zeyl and de Visser [ 36] conducted mutation accumulation tests and so were able to employ the more robust Bateman-Mukai method which gave estimates for U of ~0.003 and ~0.02 for WT and mutators respectively: these are comparable to our respective estimates of 0.0015 and 0.016 for these WT and mutator strains.
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It is worth to note that in the evolution strategy the number of children produced in one generation is not limited and it is not necessary to conduct mutation operations as in genetic algorithms.
Molecular genetic testing for mutations predisposing patients to develop cancer currently comprises of conducting mutation screening and presymptomatic testing by means of sequence analysis.
The majority of studies conducting mutation spectrum analysis use different and much shorter mutation reporter genes (cII, 294 bp; gpt, 456 bp; lacI, 1080 bp) because they are small enough to be sequenced using Sanger sequencing.
Moreover, we conducted p53 mutation analysis and revealed a mutation at codon 273 which led to the replacement of arginine by histidine.
We conducted a mutation analysis to determine whether a genetic mutation was present in the region of E2F-5 associated with the binding of transport proteins to affect the intracytoplasmic localisation of E2F-5 probservedserved in the breast cancer subtypes.
Based on this, we wanted to determine whether ATR germline mutations are involved in susceptibility to breast and/or ovarian cancer, and conducted a mutation analysis of all 47 coding exons and exon intron boundaries in the affected index cases in 126 families.
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