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All reprogramming culture steps were conducted in tissue culture plates that were tightly wrapped in Saran wrap.
However, growth curves conducted in tissue culture of pairs of ORVs and OSVs demonstrated no differences in growth kinetics or final virus yields.
However, the studies examining the changes in global and gene-specific DNA methylation patterns and histone modifications induced by exposure to Ni compounds have only been conducted in tissue culture model systems.
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Moreover, since most studies aimed at elucidating PiT1 regulation and function were conducted in tissues in which Pi per se plays an important role (i.e. mineralized tissues), very little information is available for other tissues.
To determine whether this mechanism remains functional in old mice, a step by step analysis of HSF1 activation was conducted in tissues taken from mice 2 h post-dosing with HSP990 (12 mg/kg).
Analyses of element age were conducted in two tissue groups: (1) merged villi (placental villi from all three gestational ages) and (2) merged somatic tissues (fetal brain, fetal kidney, fetal muscle and adult blood), since DNA methylation of each subfamily of Alu or L1 did not differ between the tissues included in each of these groups (data not shown).
Research on developing functional polymeric scaffolds has been conducted in diverse tissue engineering applications for a few decades.
Immunohistochemistry conducted in cerebellar tissue from unc5c −/− and +/+ mice revealed no differences in the labelling pattern (Fig. S1D).
In addition, validation was conducted in another tissue.
Detection of apoptosis and necrosis are further conducted in liver tissue and hepatocytes.
However, all of these studies were conducted in whole tissue models of inflammation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com