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The flask experiments were conducted in duplicate to ensure the credibility of results.
The procedure was conducted in duplicate to obtain free particulate organic matter (POM) and occluded POM of the light fraction (<1.8 g cm-3) and fraction of particles <20 μm of the heavy fraction (>1.8 g cm-3) [42].
The complete experiment was conducted in duplicate to obtain data for cages with mice failing to breed, cannibalism, death or injury.
The tests were conducted in duplicate to verify results.
The procedure was conducted in duplicate to obtain free particulate organic matter (POM) and occluded POM of the light fraction (<1.8 g cm-3) and fraction of particles <20 μm of the heavy fraction (>1.8 g cm-3) [ 42].
Similar(55)
The experiments were conducted in duplicates to eliminate any kind of error.
All the experiments were conducted in duplicate at 25 °C in a 100 mM phosphate buffer at pH 7.8.
All the experiments were conducted in duplicate at 37 °C in 50 mM HEPES buffer at pH 8.1.
Using commercially available kits for the measurement of human sFlt-1, PlGF, free VEGF and sEng (R & D Systems, Minneapolis, Minnesota, USA), two-site enzyme linked immunosorbent assays (ELISAs) were conducted in duplicate according to the manufacturer's protocol.
The amplification was conducted in duplicate according to manufacturer's instructions.
The amplification was conducted in duplicate according to manufacturer's instructions using 10 μM of each primer.
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