To replicate the test, genome-wide comparison between M and S forms was conducted for pools of 20 M-form and 20 S-form mosquitoes from each of two other sites that also exhibit high frequencies of M/S hybrids, at Sedhiou in Senegal and Antula in Guinea-Bissau.
aAnalysis were conducted for the pooled data for men and women, those with CMD at baseline (GHQ >2) being excluded from the analysis.
aAnalysis were conducted for the pooled data for men and women, N = 1042, those without CMD at baseline (GHQ < 3) being excluded from the analysis.
Thirdly, analyses were conducted for all questions pooled together and not for each question separately, as in the present study.
To maximize the amount of fully double-stranded PCR product, the reactions were 'chased' with additional cDNA amplification primer (1 μl of 10 μM stock) and incubated at 78°C for 1 min, 65°C for 1 min, then 68°C for 3.5 min. Multiple PCR reactions were conducted for each library, then pooled and purified using the Qiaquick PCR Purification kit (Qiagen, CA, USA).
With RevMan 5.1 software, subgroup analyses were conducted by pooling RRs for subgroups in each trial.
A comparison of the risk of death in the SVR group vs the non-SVR group was conducted by pooling the HRs for mortality.
This analysis was conducted for the total effective pollen pools and for the separate effective pollen pools originating from inside and outside of the study plot.
Data were analyzed using Review Manager (RevMan 5.0.16; Cochrane Library software, Oxford, U.K .. Separate pooled analyses were conducted for any diabetes, type 1 diabetes, and type 2 diabetes using the generic inverse variance fixed method.
Global, simplified analyses of gaseous-hydrogen releases from a high-pressure vessel and liquid-hydrogen pools are conducted for two purposes: (1) establishing order-of-magnitude values of characteristic times and (2) determining parametric dependencies of these characteristic times on the physical properties of the configuration and on the thermophysical properties of hydrogen.
