The experiments conducted comprised of: first, establishment of CCNA1 MSP; second, identification of the methylation status and correlation with expression in CC cell lines, normal cervix and CC; and finally, investigation of the frequency of methylation in normal tissues, high-risk HPV-associated low SILs, high SILs, microinvasive and invasive squamous cell CC.
Four service user focus groups were conducted comprising a total number of 22 participants; 16 people with COPD and six informal carers.
A cross-sectional survey was conducted comprising a large representative sample of 17 599 students in eight cities of China.
Six FGDs were also conducted, each comprising groups of at least six CHWs.
Experimental controls for degradation in simulated colon conditions were conducted in two sets, comprising of the disulphide cross-linked polymer incubated in Sørensen's phosphate buffer alone without presence of bacteria and incubation of B. fragilis suspension in buffer alone without the polymer.
Ten independent in vitro polarization experiments were conducted for Figures 4 6, comprising of at least one control (Het) and two test genotypes (PSdko, Rko or PSRtko) per experiment.
All the value expressed as mean ± SE. aSignificant compared with male healthy subjects, P = 0.0004 bSignificant compared with male healthy subjects, P = 0.0002 cSignificant compared with healthy subjects, P = 0.0001 dSignificant compared with healthy subjects, P = 0.0003 A blind test was conducted on 68 subjects comprising of 26 stone formers and 42 healthy subjects.
The actual assessment of the DRC's mines was conducted by groups comprised of representatives from the United Nations, business groups and the Congolese government and civil society.
Four 2 hour focus groups were conducted and were comprised of 5 7 individuals each.
The FGDs were conducted by teams comprised of both clinical and nonclinical researchers with experience in qualitative research methods.
