The ability to conduct forward and reverse genetic screens in animal models such as C. elegans remains to be one of the most powerful experimental paradigms for understanding molecular pathways underlying human disease phenotypes [ 62].
For each, control reactions were conducted without reverse transcription and without template.
We examined the reference lists and conducted a reverse-citation search of all included studies.
Other options called for shrinking the number of bases, establishing benchmarks that would mark the Iraqis' progress toward political, economic and security goals and conducting a "reverse embeds" program to attach Iraqi soldiers to American squads.
We conducted a reverse genetic screen utilizing morpholino oligonucleotides previously designed and validated against ten known zebrafish Notch pathway members [15] [19].
Reverse transcription was then conducted with a reverse primer (5'-GACTAGCTGGAATTCGCGGTTAAA-3') and Superscript II (Invitrogen).
Reverse transcription was conducted using the reverse transcriptase M-MLV from Promega.
Reverse transcription was conducted using a reverse transcription kit with an anchored oligo [dT] primer supplied by AbGene Ltd, using 1 μg total RNA in a 96-well plate.
China's central bank conducted a reverse repurchase agreement injecting a sizable 40 billion yuan into the market.
A two-step protocol was conducted with reverse transcription with gene-specific primers, followed by real-time qPCR with designed TaqMan® major groove binder probes.
