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To understand the role of known defense genes in NHR of Brachypodium against switchgrass rust, we conducted a transcript profiling of defense-related genes in Bd21 leaves at various time-points after P. emaculata inoculations.
Kimmig and co-workers, working in Peter Walter's lab at UCSF, conducted a transcript analysis of ER-stressed S. pombe cells and found a distinct decrease in the abundance of mRNAs encoding for proteins that were destined for the ER (Kimmig et al., 2012).
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Finally, to investigate the role of alternative splicing (AS) in response to salt stress, we conducted a survey of transcript isoforms across the four libraries and examined six common types of 'alternative splicing events'.
In order to explain the variations in resistance in Bd3-1 and Bd30-1, wextendeded our analysis by conducting a basal transcript profiling of tested defense-related genes in Bd3-1 and Bd30-1 in relation to Bd21.
Some of the authors conducted a thematic analysis of transcripts from one programme area each to extract factors influencing screening uptake.
Following the workshop, we conducted a metanalysis of the discussion transcript.
To further advance our previous findings, we conducted a detailed and comprehensive global transcript analysis in biofilms at distinct developmental stages using cDNA microarray in conjunction with a computational tool (MDV) for data mining.
To further validate if there were more than one transcript we conducted a cDNA blot using mRNA from different developmental stages.
To determine taxonomic origin for each transcript, we conducted a series of BLAST searches and filtering steps outlined in Figure 1.
The lead researcher conducted a close reading of each transcript individually and coded them for basic important themes that emerged from the interviews.
We conducted a thematic analysis of interview transcripts to identify benefits, drawbacks, and barriers to routine collaboration on PLA negotiations.
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