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JS, RHJ, AM, JML, ML-M, and CG have been consultants for ATI since 2010 or 2011 and have received payment for services not related to the conduct of the Pathway CH-1 study.
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Gene Ontology (GO) analysis was conducted of the pathways significantly (P < 0.05) enriched in the differentially regulated genes (Additional file 6: Table S3).
To determine over-representation of the transcripts and metabolites in the 351 pathways in MetNet and the transcripts in Gene Ontology categories, separate analyses for genes/metabolites were conducted for the pathways and for each of the three ontologies molecular function (1,053 categories), biological process (1,787 categories), and cellular component (381 categories).
In a next step a cluster-randomized evaluation study according to the MRC-model [ 26] should be conducted to assess the effectiveness of the pathway.
For the conduct of pathway analyses, we first identified a set of 'independent' signals providing nominal evidence association (using an arbitrary P-value criterion of <10−2) for each of the two disease traits using LD pruning.
Future work should refine the description of the pathway and conduct a more comprehensive analysis of currently available data.
A final re-analysis of the pathway was conducted to ensure all objects were still connected to the main pathway.
The evaluation of the pathway was conducted in a qualitative design on a sample of 12 pathway developers (8 GPs and 4 cardiologists) and 4 pathway users (GPs).
Numerical integration of the pathway was conducted in a two step process, with all the fluxes f → (t ) being calculated from metabolite concentrations and then all the metabolite concentrations being updated from the net fluxes.
Study on an investigation of the importance of the pathway has been conducted by deleting the calcineurin regulatory subunit, calcineurin b1 (Cnb1).
Our result should encourage the conduct of studies to identify pathways affected by genetic variation that are associated with postpartum haemorrhage, which could result in new insights into the pathogenesis of this complication.
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