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The tandem instrumentation used in these studies utilized two or three discrete drift tubes and time-depletion ion gates in order to conduct mobility-selective experiments.
The coupling of multiple stages of ion mobility dispersion provides a means of conducting mobility-selected experiments.
Additionally, CloudExp conducts various mobility scenarios for mobile devices.
To conduct electrophoretic mobility shift assay (EMSA) analysis and ELISA, the liver was immediately snap-frozen in liquid nitrogen.
The article reports on original empirical work conducted with mobility scooter users in a car dependent city, and the ways they negotiate a fossil-fuelled transport system designed for automobiles.
To determine if the CREB element can regulate PP2A-Aα promoter, we also conducted gel mobility shifting assay with an oligo primer containing the CREB binding site derived from the PP2A-Aα promoter (Fig. 4A).
To test if AP-2α also regulates PP2A-Aα gene promoter, we again conducted gel mobility shifting assay with an oligo containing the AP-2α (1) binding site (Fig. 5A).
To elucidate if SP-1/SP-3 regulates PP2A-Aα promoter, we also conducted gel mobility shifting assay with an oligo containing the SP-1/SP-3 binding site from PP2A-Aα (Fig. 6A).
To assess the effect of USP7 on p53 DNA binding, we conducted electrophoretic mobility shift assays (EMSAs) using a Cy-5 labeled consensus p53 binding sequence as a probe and a version of p53 spanning the core DNA binding and the C-terminal regulatory regions (p5382 393) but lacking the transactivation domain, (Figure 1A).
An independent physiotherapist (not involved in the instrument development) conducted the mobility assessments.
SLDM conducted electrophoresis mobility assays, statistical analyses, and drafted the manuscript.
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