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The solvent extraction of biosurfactant compounds produced by ST34 and ST5 was conducted as outlined in Vosloo et al. (2013).
RNase treatment of extracts was conducted as outlined [62].
Measurements were conducted as outlined by American Thoracic Society guidelines [29].
Colony formation assays were conducted as outlined previously [ 18- 20].
FDR calculations were conducted as outlined in Storey and Tibshirani (Storey & Tibshirani, 2003).
An acute adult lethality test was conducted as outlined by OECD Technical Guide 203 [ 21].
Bombardment assay was conducted as outlined below, modified from Chiou et al. [ 14].
Data analysis was conducted as outlined in the statistical analysis plan utilizing SAS software version 9.1.3 (Cary, NC, USA).
The MTC cDNA preparations utilized for RT PCR analysis were conducted as outlined by the manufacture in a final reaction volume of 20 µl.
To assess the impact of blended CBT for depression compared with CBT as usual on health care budgets, a budget impact analysis (BIA) will be conducted as outlined by Mauskopf et al. [ 32].
To assess how health care budgets are changed by offering bCBT for anxiety compared to fCBT, a budget impact analysis (BIA) will be conducted as outlined in Mauskopf et al. [ 58].
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