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Proteins from equal volumes of cytosol from the various experimental conditions were resolved by SDS-PAGE and analyzed by immunoblotting with antibodies specific for CCT- eta, CCT-beta, and α-SMA.
Proteins in non-reducing conditions were resolved on 8% polyacrylamide gel containing 1mg/ml gelatin (Sigma).
After several approaches in which we tried various antibodies, incubation times, blocking reagents, epitope extraction methods and chromogen stains, conditions were resolved where autophagosomes were clearly identifiable and distinguishable from nonspecific staining artifacts (Fig. 1B).
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Generally, models with parameterized convection reproduce the temperature profiles of the LES reference accurately when the surface boundary conditions are resolved by the grid.
For example, if you are choosing between charities aimed towards curing blindness vs. HIV, you must consider two things: the prevalence of the two conditions and the potential quality of life improvement if either condition were resolved.
Total cell lysates from equivalent cell numbers of each tested condition were resolved by 10% SDS-PAGE.
Cell lysates from each condition were resolved by SDS polyacrylamide gel electrophoresis (PAGE) and subjected to western blotting with mouse monoclonal anti-FLAG and mouse monoclonal anti-Hsp60 antibodies.
Until the condition is resolved, I bear with it".
Although this condition is resolved after termination of pregnancy, subtle metabolic changes can remain and prompt incidence of metabolic syndrome.
The unstable occlusal condition was resolved in a few months, and orthodontic treatment was completed 7 months after surgery.
Patients in whom IAH was not resolved (57.6%) had a significantly higher mortality rate than patients whose condition was resolved (64.7 vs. 35.2%, p = 0.001).
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CEO of Professional Science Editing for Scientists @ prosciediting.com