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The ultrasonic experiments using the optimum conditions were replicated three times and the results were compared with the predicted values for validation of the model.
To understand the events during the pressure spike during the first few tenths of a second, the experimental conditions were replicated approximately using a displacement-controlled materials testing machine.
In order to determine at what concentration protein aggregation is induced by the varying salt solution conditions, the protein hydrodynamic radius was tracked using DLS measured particle size under identical buffer conditions (although not all conditions were replicated as at some of the higher ionic strengths aggregation proceeded too rapidly to accurately measure using DLS).
To rule out the possibility that the force of agonist injection could cause cells to release ATP, aequorin assay conditions were replicated for Ax2 cells in the presence of luciferase luciferin (ATP bioluminescent assay kit (Sigma)) according to the manufacturer's instructions.
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It may further be questionned whether elevated, but low levels of muscle activity in FM patients relative to healthy controls (HC), observed under strictly controlled laboratory conditions are replicated in situations close to normal living.
The wells for each experimental condition were replicated five times and the representative results from three individual experiments were shown.
The methodology can be used even with the most minimal levels of replication, provided at least one phenotype or experimental condition is replicated.
Second, each condition was replicated 100 times, which was relatively small in simulation studies.
Levels of each factors are fully crossed to create 864 conditions, and each condition is replicated 100 times.
Each condition was replicated twice.
Each condition was replicated in five wells.
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