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Occlusal conditions were assessed using latex gloves, mouth mirrors, callipers and millimetre rulers.
Loading conditions were assessed using two different rear securement point positions.
The effects of operating conditions were assessed using the developed model.
The SFE conditions were assessed using a general full factorial design; the experimental factors were pressure (15, 25, and 35 MPa) and temperature (40, 50, and 60°C).
Validation criteria such as specificity, detection limit, accuracy, reproducibility and storage conditions were assessed using reference plasma samples prepared as DPS and clinical DSS from the German molecular HIV-1 surveillance processed under real-life transportation conditions.
The effects of the UCMS and fluoxetine in both diet conditions were assessed using physical (coat state and body weight) and behavioural tests (the reward maze test and the splash test).
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Differentiation potential in the UTA1 cells grown under KSR- and hESF9a-based conditions was assessed using in vitro differentiation assays involving embryoid body generation.
Aggregation of human platelets under high shear conditions was assessed using a standard optical aggregometer (Lumi- Dual Aggregometer; Chrono-Log, Havertown, PA, USA) at 37°C and 1200 rpm in a volume of 500 mL buffer with a final platelet concentration of 6.5×105 platelets/ml over a 5 min interval.
The importance of vascular effects under the same treatment conditions was assessed using fluorescein angiography.
Statistical significance of the difference between the different conditions was assessed using a two-tailed Student's t-test.
The association between T2DM and comorbid conditions was assessed using logistic regression adjusting for age and sex.
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