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The experimental conditions sequence was random.
In these conditions, sequence alignment between strain 2-22 A909A909 genomes revealed 621 simple insertion/deletion events ranging from 1 to 10,095 nt.
Shown are representative data time traces and corresponding simulated traces for three conditions: sequence 1 at 12 pN (red), sequence 2 at 12 pN (green), and sequence 3 at 9 pN (purple).
Under standard amplification conditions, sequence sections exclusively composed of GC residues will terminate elongation steps in PCR by forming highly stable stems or GC clamps causing amplification to be refractory.
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This is done, using ladder logic diagram, statement lists, or control flowcharts software, by representing the logical conditions, sequences, and interlocks required for operating equipment or a machine in an automatic sequence.
Amplification conditions, sequencing reactions, clean-up, and sequence visualization followed Unmack et al. [ 51].
The experimental conditions, sequences and other relevant data are easily downloadable in different formats.
PCR primers, reaction conditions, sequencing primers, and target genomic sequences are summarized in Additional file 1: Table S1 and S2.
Full details describing primers, PCR conditions, sequencing, alignment, and substitution model priors can be found in [ 96].
The average background RPKM of 0.13 was found to be in a similar range compared to the eleven conditions sequenced with the SOLiD technology.
The modified boundary condition sequence is then applied to control die/node separation during the backward deformation simulation.
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