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The experimental conditions of expression and purification of each recombinant protein were optimized.
Expression patterns provided further information on conditions of expression of a number of sRNA candidates.
Amplifications were performed by two operators in two different laboratories in order to mimic "real experimental conditions" of expression profiling.
Expression patterns derived from microarray hybridizations provided further information on conditions of expression of a number of sRNA candidates.
After transformed in competent Escherichia coli K12 strains were fermented in batch and fed-bach mode in different mediums in order to select the best conditions of expression.
This was performed with the VC-AbdA construct: intensities of the GFP fluorescent immunostaining were measured and compared to embryos expressing VC-AbdA with the arm-Gal4 driver in order to adjust the physiological conditions of expression.
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We used NiCo21 (DE3) to express 6His-tagged HIV-1 CA using shake flask culture condition of expression.
The first set of experiments aim to measure the efficiency of face recognition for identical twins and non-twins under the condition of expression variation.
Initial experiments with the wild-type PfI2 cDNA did not allow the production of recombinant protein whatever the bacterial plasmid and the condition of expression used.
Furthermore, FM1 medium, a solution of yeast extract, glycerol, soy peptone, minerals and vitamins, was used in the same conditions for feed-batch and batch fermentation in order to investigate the best condition of expression.
These methods use linear fragments and search through combinations of conditions for expression of activity, and were used to extract common substructures in dopamine agonists [13].
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