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HEPES is a Good buffer for maintaining physiological conditions in cell cultures but poses some problems like long-term stability especially when exposed to humid air and light.
These two MAPKs are reported to activate MSK1 in inflammatory conditions in cell lines like HEK293 and L929sA [7].
For this reason, we carried out experiments in three different conditions:in cell lysates, in live cells and in whole animals.
To analyze cellular migration and adhesion, cells induced with doxycycline for 8 days were seeded under subconfluent conditions, in cell culture plates or coverslips, for 20 h followed by 4 h serum starvation.
Using selective TG2 inhibitors and tagged nucleophilic amine substrates, we show that the majority of extracellular TG2 is inactive under normal physiological conditions in cell culture and in vivo.
Studies have shown that treatment with CoCl2 can be used to artificially induce hypoxic conditions in cell culture [ 25].
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In cell-free protein engineering, the direct control of reaction conditions in cell-free system allows for easy synthesis of complex proteins, toxic proteins, membrane proteins, and novel proteins with unnatural amino acids.
However in real LTE networks, UE association criteria also include factors such as UE velocity, load conditions in cells, and backhaul capacity.
Recent individual cell studies have indicated that p53 activation is highly regulated in response to stressed conditions and non-stressed (normal proliferating) conditions in cells.
The capacity to recreate these disease conditions in cells in the lab will help researchers look for new drugs.
In cellular model, ΔΨm loss and cytochrome c release were simultaneously detected in response to ABT-737 [39], [39] contrary to what was observed with our conditions in cell-free system.
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