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For these conditions, hereafter referred to as the 'high Te_outer regime', experimental points denoted by dotted lines in Figure 1a were produced, indicating a change in the behaviour of the accelerated electrons compared with the standard regime.
EP-PCR reactions were carried out under the following conditions, hereafter referred to as standard: 10 ng of DNA template, 10×PCR buffer (Mg2+ Free, TaKaRa), 10 mM of each dNTP (dATP ∶ dTTP = dGTP ∶ dCTP = 1 ∶ 5), 10 µM of both primers (VHForNcoI and VLRevHindIII), 25 mM MgCl2 and 2.5 U Taq DNA polymerase (TaKaRa) in 50 µl volume.
For characterization of the global transcriptional patterns of B. cereus G9241, B. anthracis Sterne 34F2, and B. cereus 10987 (hereafter referred to as G9241, B. anthracis, and 10987, respectively), we first determined the growth characteristics of these three strains in a semi-defined medium under both high aeration and 14% CO2+0.8% bicarbonate conditions (hereafter referred to as O2 and CO2).
Specifically, its distribution might reflect a wide tolerance owing to a geographically unstructured, generalist phenotype that is adapted to a broad range of conditions (hereafter, generalist phenotype strategy).
Alternatively, the species may be characterized by local phenotypes, product of plasticity or local adaptation to local environmental conditions (hereafter, localized phenotype strategy).
Options should always be informed by evidence about local conditions (hereafter referred to as local evidence) together with other forms of evidence.
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This condition, particularly referred to as the Backus' FF condition hereafter, is only necessary and not sufficient for r to be exclusively generated by advection (Gubbins, 1984).
To evaluate free-surface condition, we performed another SCM simulation in a full-space elastic medium, so as to remove the free-surface condition (hereafter, we call it SCMF).
For all future experiments we used 20 mM D-glucose as our repressing condition (hereafter, 'glucose' plates) and 10 mM L-arabinose as our inducing condition ('arabinose' plates).
Equation (1) leads to various necessary conditions (we hereafter refer to them as 'FF conditions') that have to be satisfied by radial MF and SV at the CMB (Backus, 1968).
aCD4 that were activated overnight as described above were collected, washed, and cultured in 24-well plate at 1 × 10, 2 × 10, or 4 × 105 cells/2 ml of CM for 48 hr to generate conditioned media (hereafter called 5 × 10, 1 × 105 and 2 × 105 aCD4S, respectively).
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