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Boundary conditions for primary combustion product composition were obtained from the fuel bed by experiments.
To produce a tool for cellular and molecular studies in genetically engineered murine models, we defined the optimal culture conditions for primary cultures of articular chondrocytes from newborn mice (C57Bl/6).
That the ECB would buy from banks, not governments, makes little difference because it would still affect yields and thus the conditions for primary bond issues.Moreover, if debtor countries defaulted, the ECB would make losses and would call on its 17 shareholders, the central banks of euro-zone countries, to pay.
Conditions for primary antibody hybridizations were followed according to the antibody datasheets.
Based on these results and those of several other trials, we determined the optimal incubation conditions for primary antibodies (flow rate, 100 µl h−1; incubation period, 10 min), which translate into a >6-fold decrease in required time and 200-fold decrease in antibody consumption.
RT-PCR conditions for primary MIR398b and MIR398c transcript amplification followed the method described by Sunkar [ 35].
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Our approach, although it avoids the problems associated with using whole ovary as a normal sample, has the limitation that we do not know whether and which changes in microRNA expression may be attributed to culture conditions used for primary HOSE cell culture.
The immortal DF-1 CEF cell line was grown using the same conditions as for primary CEF cells.
The discrepancy between these two mouse models might be due either to different genetic background or to diverse experimental conditions used for primary cultures of neurons.
The set of equations and boundary conditions for the "primary potential/current distribution" after a small-amplitude potential step has been analyzed for a film-coated disk electrode in contact with an electrolyte.
Combining O*NET with the CPS allows us to account more directly for occupational skill requirements, job tasks, and working conditions for both primary and secondary jobs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com