ChIP was performed as described elsewhere [4], [48] using varying cross-linking conditions depending on the assay (conditions available upon request).
Each of the target genes were real-time amplified from cDNA using oligonucleotides specific to each gene (sequences and conditions available upon request), and Rps29 was used as the normalization control.
PCR was performed as a nested hot-start PCR (conditions available upon request) with the primers listed in Table 1.
Primers were first tested with genomic DNA as a template, and then with whole ovary cDNA as a template (primers and conditions available upon request).
Following immunoprecipitation with anti-MYB and IgG control antibodies, the MGMT promoter region was amplified with specific primers (primer sequences and PCR conditions available upon request).
Confirmatory analyses and familial studies were done by microsatellite marker segregation analyses and commercial or home-made MLPA studies (primers and conditions available upon request).
