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The initial mobile phase composition was 9.0% A, which was held for 2 min, then increased to 20% A at 4 min, then held for 6 min. The flow rate was increased to 0.20 ml min−1 at 10.5 min, then to 0.25 ml min−1 at 12.5 min in order to speed conditioning of the column back to the initial mobile phase composition of 9.0% A. At 13 min, the flow rate was decreased to the initial rate of 0.175 ml min−1.
Similar(58)
It was hypothesized that the pre-conditioning step with acetone generates a background signal after Strata SPE cleanup, while for the self-packed columns, the acetone is removed during drying at 50 °C after conditioning of the columns.
Tetrasodium salt of ethylenediaminetetraacetic acid (EDTA) is used in the conditioning of cadmium column to increase the column life by preventing the formation and coating of Cd (OH 2.
A sensitivity analysis of the column ill-conditioning vs reflux ratio, number of stages and operating pressure is presented in order to relate the engineering guidelines to control considerations.
The digest was concentrated on an Agilent Bond Elut Plexa SPE column (60 mg): After conditioning the column with 1 mL methanol and 1 mL 5 % formic acid in water, the sample was applied onto the column.
The hot utility for the feed conditioning of columns 1 and 2 has been counted in Table 9.
After pre-conditioning the column with 6 mL hexane, it was ready for use.
"It's the conditioning of the golf courses," he said.
But it must be conditioning of the right sort.
Conditioning of the environment of the learner.
Myeloablative conditioning of the brain itself had no beneficial effect.
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