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During habituation to the conditioning compartment, a single direct experience of the UCS resulted in elevated output from the heart in both BALB and B6 mice (maximum HR increase±s.e.m.; +40±8 and +23±9 bpm for BALB & B6 mice, respectively), which peaked ≈120 s after the UCS was applied (Fig. 7).
The second group of rats (fear-potentiated) received three footshocks 0.3 mA during 3 s separated by 30 s interval in a conditioning compartment on the first day.
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or vehicle and were immediately confined into one of the two conditioning compartments for 30 min. A combination of unbiased and biased allocation was used.
The light intensity settings were set at 30 (80 lux) within the conditioning compartments and 90 (380 lux) in the central compartment, with these values referring to the settings on the equipment provided.
There was no difference between time spent in each of the conditioning compartments during pre-test (genotype x compartment interaction: F 1,31) = 1.3; p = 0.26), ensuring an unbiased baseline (Fig. 9A).
Rats were placed in the interim chamber of the apparatus with the doors opened and allowed free access to the conditioning compartments for 15 min. The time they spent in each compartment was recorded during this 900 s drug-free test session and used as the preference score.
On day two, each rat explored one of the conditioning compartments for 30 min. Two hours after the CFA injection, each rat was allowed to explore the second compartment for 30 min. On day three, the time spent by the rats in each compartment was recorded again.
Cannulated mice were trained in a balanced, three-compartment conditioning apparatus as described [1].
Then the entire system which includes air conditioning unit and the compartment are studied.
The rats showed place aversion (i.e. affective pain) by spending less time in a pain-paired compartment after conditioning than during a preconditioning test.
In the CPP test, lowering the association between amphetamine and the initially non-preferred compartment after conditioning decreases the drug-seeking response (Ninkovic & Bally-Cuif, 2006), verifying contingency degradation.
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