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To do this, my colleagues and I presented rats with a tone followed by a mild electric shock in a conditioning box.
The environmental conditions, i.e., temperature and relative humidity of electrospinning process were systematically changed using a high accuracy conditioning box to provide a comprehensive investigation of morphology evolution within electrospun fibers.
Six laps later, Biffle reported that the cool box (air conditioning box) will have to be replaced during their first pit stop.
Wild type mice froze immediately when they were placed in the conditioning box.
After each daily 30 min session in the conditioning box, the animal was returned to the dark sound attenuated chamber and we recorded LFPs and EMG for 4 hours.
The animal was then moved to a stainless steel and Plexiglas conditioning box (30×22×19 cm) with a shock grid floor for 30 min, and then finally placed back in the recording chamber for 4 hours of spontaneous LFP recording.
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Animals were individually placed in standard conditioning boxes (see above).
Context fear conditioning was performed as previously described [31] using Med Associated Mouse Context Conditioning boxes.
Conditioning boxes.
After the initial handling period, all rats in this experiment were given 3, two-day training trials in the conditioning boxes.
Three sets (transmitter and receiver) of infrared photocells were spaced equidistantly along the long wall of the place preference conditioning boxes (2 cm above the floor) in order to record the time spent in each conditioning chamber.
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