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To facilitate extinction, rats will be administered an HDAC inhibitor (HDACi) following nonreinforced exposure to the conditioned context.
The freezing response on hearing the tone is evaluated in both the novel and conditioned context (Salehi et al., 2009).
It is shown by both previous studies (Rudy and O'Reilly, 2001) and our data that animals without pre-exposure or pre-exposed to an irrelevant context do not display freezing behaviors to the conditioned context.
They also suggest that under our experimental conditions the 1st reexposure to the aversively conditioned context engages extinction processes that does not depend on NMDA/NO neurotransmission in the vMPFC at the time of reexposure.
We showed in a similar contextual fear conditioning procedure that a 2 min exposure to a conditioned context engaged reconsolidation processes which stabilise or maintain the fear memory for subsequent recall, as measured by high levels of conditioned freezing at all recall tests [24].
The following day, the mice are tested for contextual conditioning by being reintroduced to the initial chamber (conditioned context) and being exposed to the tone, again without receiving the shock.
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During extinction training each rat simply received a 2 min exposure to one of the conditioned contexts and a 10 min exposure to the other conditioned context.
One or two days after contextual fear conditioning, rats were re-exposed to one of the conditioned contexts either for 2 or 10 min. 24 hours later the rats were exposed to the other conditioned training contexts for either 2 min or 10 min. The order of the conditioned contexts that the rats were exposed to during extinction training was counterbalanced.
The rats were placed into one of the conditioned contexts for 2 min and the following day they were exposed for 2 min to the other conditioned context.
Extinction training: Each rat received two extinction training trails in the two different conditioned contexts separated by 24 hours.
(a ) Recall of the fear conditioned (FC) context is measured by the percentage of freezing when re-exposed to the fearful context.
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