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We conditioned a total of 180 individuals, 12 fish per day.
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Following that change in operating conditions, a total of five chemical cleanings were carried out using a standard cleaning solution.
In our study with the low aeration condition, a total of 487 genes showed significantly different expression levels (fold change ≥2) in C2, with 281 upregulated and 206 downregulated genes (Additional file 1: Table S3).
In the mismatched condition, a total of 170 tool transfer events were observed.
Each participant was shown a randomly pre-selected subset of stimuli for each of the three conditions – a total of 210 stimuli.
In a biological replicate performed with similar amounts of sample and with similar FGF-2 stimulation conditions, a total of 574 tyrosine phosphorylation sites could be identified.
Under these conditions, a total of 1324 spots were clearly detected and subsequently analysed using Delta2D software for differential protein expression.
Although all birds pecked the worms in the control condition, a total of nine birds did not peck the worms in the test condition within 10 min (five hand-reared, four wild-caught).
Under these conditions, a total of 515 genes hyperactivated the reporter in at least one DNA concentration following heat shock at either 43 or 44°C and at either time point (Fig. 2).
For the 6 SNR levels of the A and the A = V conditions, as well as the V-alone condition, a total of 520 words were drawn from the same pool (6×2+1 = 13 combinations of condition and SNR level; 40 trials each; 13×40 = 520).
For each cell line exposed to each condition, a total of 30 replicates were collected.
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