Of the six up-regulated genes in the conditional experiment, four (Ly6a, Tbx3, 1700097N02Rik, 2210409E12Rik) were also found amongst the 34 constitutively up-regulated genes.
Affymetrix gene chip analyses were performed with mouse Set430_A arrays for the constitutive experiment or mouse Set430_2.0 arrays for the conditional experiment.
Notably most of the down-regulated genes in the conditional experiment are expressed from CpG island promoters (48/57), particularly in the group that overlapped with the constitutive experiment (23/24), which are most likely to be directly regulated by Mll2.
The constitutive experiments were performed in triplicate whereas the conditional experiments were performed in quintuplicate.
However, future CMCM-mediated conditional experiments could be performed at the fitted transgenic gRNA loci via the phiC31-mediated attP/attB system (Ni et al. 2008).
In both experiments, Wilcoxon's signed ranks tests showed that, as we expected, participants used subjunctive constructions to paraphrase counterfactual conditionals more often than indicative conditionals (Experiment 2a: 36% versus 4%, z = 3.72, N − Ties = 20, p < .001; Experiment 2b: 39% versus 13%, z = 3.9, N − Ties = 24, p < .001), as Table 3 shows.
Results from this conditional knockout experiment show that in the non-AAV-Cre-injected contra-lateral side of the ARC, c-Fos immunoreactivity (Figure 5C) and POMC mRNA expression (Figure 5D) were increased after i.p.
After consultation, Michel Nussenzweig, our Reviewing editor, has decided that the conditional KO experiment suggested by referee #3 is not necessary.
Data from both conditional knockout experiments and in vitro experiments using MSCs suggest that FGF signaling may act as a balancing factor to maintain the size of the skeletal progenitor pool while avoiding overgrowth, by stimulating stem cell proliferation while inducing committed progenitor differentiation.
1. Conditionals for Experiment 1a and 1b presented with the additional enabler (conditionals marked with * were used in Experiment 1b ).
Mixed backcrossed animals were used for the global Snrk KO experiments and non-backcrossed animals were used for all of the conditional null experiments.
