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For P1 expression analyses of inbred and landraces lines under control and UV- B conditions, nested RT-PCR was performed as previously described [ 54].
The last condition (nesting condition) forbids crossing base pairs, i.e. pseudoknots.
Four types of driving demands (baseline, narrow lane, pace clock, combined) were crossed with three levels of message condition (no message, paragraph, parsed), and message condition was nested by two trigger points (far, near), resulting in five combinations (only paragraph and parsed conditions were crossed with trigger points).
Sequences of primers and conditions of nested PCR are available upon request.
Almost all potential distributions proposed by CCSM conditions are nested within the MIROC projections.
This study's data set was longitudinal because multiple observations for medical conditions were nested within the respondents.
The study employs a longitudinal dataset because multiple observations over various medical conditions are nested within an individual.
Conditions for nested PCR analysis of each marker were similar to those described (16 – 18 ), except for differences in annealing temperatures specified in Table 1.
PCR conditions for nested PCRs comprised a pre-denaturation step at 94°C for 2 min, followed by 40 cycles each with denaturation at 94°C for 1 min, annealing at 60°C for 1 min and extension at 72°C for 1.5 min, and terminating with a final extension step at 72°C for 5 min.
M. conformis seems to tolerate a broader range of ecological conditions nesting in sunny and shaded environments of sand dunes and the workers are active during the day and night (Cardoso, pers. obs).. Yet, M. simplex exclusively nests in sunny sand dune places and its workers are active only during dusk and overnight [ 65].
Strict limitations were imposed on the selected types and parameters to satisfy the nested condition [28].
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