This data again indicated the inhibitory effect of BTB extract on both cell lines with vacuole formation, condensed mitochondria formation, dispersed chromatin, apoptotic body formation, autophagic vesicles and membrane blebbing evident, as compare to untreated cells.
Vacuole formation, dispersed chromatin, apoptotic body formation, autophagic vesicles, membrane blebbing, condensed mitochondria formation, and swollen mitochondria were noted in the BTB treated cells (Fig. 6B, C, E, F).
Fig. 6 Ultrastructural features of cell death: an 48 h exposure of MCF-7 and MDA-MB231 cell lines in BTB extract in resulted in vacuole and condensed mitochondria formation, dispersed chromatin, apoptotic body formation, autophagic vesicles and membrane blebbing (B, C, E, F) with comparison to untreated control (A, D).
The DNA condensed particle formation has been measured at UV absorbance of 320 nm [ 12].
For example, based on this criterion, 'moléculas'moleculesles') is identified as a candidate term since it is the head of a noun phrase in sentence 'Moléculas de ácido silícico condensam com formação de água' ('Silicic acid molecules condensed with the formation of water').
Within the eukaryotic cell nucleus, DNA is condensed through the formation of chromatin, a protein−DNA complex with the nucleosome as its fundamental unit.
Typical apoptotic morphological changes characterized by loss of adherence, condensed cytoplasm, and formation of apoptotic bodies were observed when VECs were exposed to TNF α.
Apoptotic cells were identified by the appearance of specific nuclear staining, condensed nucleus and/or formation of apoptotic bodies as examined at 400× magnification.
Each growth inhibition was also significantly enhanced in the presence of TRAIL as shown in Figures 3B and C. In addition, cell death induced by a combination of camptothecin and TRAIL showed clear changes in morphology that were typical of apoptotic cell death including decrease in cell size, condensed nucleus and formation of apoptotic bodies, as shown in Figure 3D.
He operated primarily out of condensed, tight-end-heavy formations, where he could set up timing-and rhythm-based throws in the short and intermediate areas.
