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Developing suitable analytical methods for the concurrent quantification of multiple exposure biomarkers is challenging because optimal conditions for the hydrolysis of such conjugates (e.g., O-glucuronides, N-glucuronides, sulfates) may differ depending on the biomarker.
This means that SWATH MS analysis, when combined with N-glycosite enrichment, provides a reproducible, deep and quantitative digital map of the human plasma N-glycoproteome, which will permit the concurrent quantification of a significantly higher number of glycoproteins than SRM from similar amounts of sample and in comparable time frames [84].
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The GC-MS method allows for the concurrent analysis and quantification of several OC pesticides and PCB and PBDE congeners, facilitating extensive data acquisition from small specimens.
Among the emerging quantitative proteomics technologies, isobaric tags for relative and absolute quantification (iTRAQ) allows concurrent protein sequence identification and relative quantification of those peptides with known protein sequences in up to eight different biological samples in a single experiment [ 20].
Kinetic quantification normally requires continuous concurrent monitoring of radioactivity in the blood.
A further problem that limits NO quantification in general is the concurrent presence of reactive oxygen species (ROS).
In fact, as was shown recently, confidence of senescence cell quantification could be improved by concurrent usage of a positive marker with a negative one, e.g. the presence of γ-H2A.X foci or SAHF/SA-β-Gal positivity and the absence of proliferative antigen Ki67 (Lawless et al. 2010).
The specificity to only a subset of all peptides renders this relative quantification method less well suited for concurrent direct protein quantification.
For accurate normalization we recommend their concurrent use since this results in more reliable quantification.
Hence, despite the well-established diagnostic accuracy of LUS for the diagnosis of pneumothorax, no evidence-based guidelines have been established on how to use LUS for the quantification of pneumothorax and the role of concurrent supplementary imaging such as plain chest X-ray or CT.
Further to monitor the differentiation profile, concurrent investigations were carried out either by the flowcytometry quantification or by selecting 5 10 random fields and counting various neural populations relative to the DAPI positive total number of cells in the medium and high density plated cultures.
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