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The concept of concurrent amplification of multiple oncogenes in breast carcinomas is not novel.
Intriguingly, none of the cases with 8q22 amplifications showed concurrent amplification of 2p25.
AR- TNBC (40% vs. 4%), and often associated with concurrent amplification of the PIK3CA locus.
Primers were designed for intron 4, intron 6, and intron 10 of PROS1, avoiding concurrent amplification of PROSP.
The concurrent amplification of mutant and normal probes is kept in balance by using the same P2 for each probe.
Second, amplification of the signal with horse-anti-mouse secondary antibody resulted in concurrent amplification of endogenous immunoglobulin, which completely obliterated the image.
Similar(51)
Frequent concurrent amplification and mutation of PIK3CA in human breast cancers.
We propose that these clonal growths would have a survival advantage on initiation of hormone deprivation therapy, and could seed the formation of CRPC, with concurrent further amplification of the AR gene by similar mechanisms.
Furthermore, future applications of multi-targeted primers may include concurrent selective amplification of DNA sequences of interest from one or multiple genomes for the high-throughput sequencing of multiple homologous loci.
The concurrent mutation and amplification of PIK3CA frequently occur in all of breast cancer with 42% (18 of 43) of tumors with amplified PIK3CA also harboring a PIK3CA mutation (Additional file 3: Figure S2).
The difference may reflect an occasional amplification of p53 concurrent with the rest of chromosome 17 due to aneuploidy.
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