In addition, 100 randomly selected specimens negative with polymerase chain reaction and 20 concordant positive samples were masked and tested by this latter assay to minimise potential bias introduced by testing discordant samples only.
Our real-time PCR identified those serotypes in the PCV-13 vaccine and gave results that were concordant with those of the Quellung reaction, and without cross-reactivity with other potential pathogens causing meningitis.
The activities of complex III and IV were also increased in the brains of double mutants relative to Sco2 KOKI animals, but remained significantly lower than those measured in Parp1 −/− and WT littermates; the histochemical reaction to COX was concordant with the biochemical data.
Average coverage of the 23 samples was at least 490X in the reactions: Most variants were concordant between two different MPS platforms and with non-coding region data generated by Sanger sequencing.
We were interested in further study of the two discrepant genotype cases, since both had previously been concordant with HapMap in the 7-reaction-multiplex PCR data set, and both showed high quality, unambiguous SNP Charts in the 50-plex PCR data set.
For each sample, methylation status is the concordant results of at least two separate bisulfite reactions and a minimum of two amplification reactions for each aliquot of bisulfite-treated DNA.
There were 1888 subjects who showed a positive SPT to at least one of six allergens with the highest prevalence of positive reactions (hazel, birch, grass, cat, dog, and Dp; the reactions to the two mites were concordant in 80%).
Of the 200 concordant records re-reviewed for concordance, all records were found concordant.
In case of non-concordant results, serotypes were double-checked using the Quellung reaction and the multiplex PCR assay.
Concordant negative galactomannan and computed tomography supported by a polymerase chain reaction assay were shown to have the highest discriminatory power to exclude invasive pulmonary aspergillosis.
