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These differential patterns of binding with nuclear extracts of the B cell line WIL2 NS were obtained in three or more concordant experiments.
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The ability of the E6 protein from different types to degrade p53 was concordant in all experiments.
Within these, 449 (87.2%) passed both quality criteria (see earlier) and were concordant in both experiments.
These findings are concordant with similar experiments in the mouse, where animals expressing a disease-linked APP mutation, with or without mutant PS1 and tau, were found to retain essentially normal DD circadian behaviour throughout life (Wisor et al., 2005; Sterniczuk et al., 2010).
Although the exact biological mechanisms are not clear, data from animal experiments are concordant with our findings.
All these recombinant sequences were subsequently corrected by de novo experiments – fully concordant with the predictions from our data-analytical approach.
The results in the cytotoxicity assays and in the enrichment experiments were concordant.
Concordant with RNA-seq experiments, ZNF777 and ZNF282 are both highly expressed in multiple cell types in both fetal and maternal components of the human term placenta (fig. 6 C and D).
This highlights the challenge in assessing performance metrics with a small set of synthetic constructs even at the highest abundance in our experiments, 100% concordant results were not obtained for all of the SFGRs.
We investigated whether we could identify concordant regulation through heterologous hybridization experiments in the other species using the 27 genes selected above (> 75% concordant regulation across clones in A. burtoni).
The protocols used in the present study allows the assessment of a minimally altered gene expression pattern that is concordant with in situ hybridization experiments (M. Rumbo unpublished data).
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